Binod Rayamajhee

PhD Candidate

Research Title: “Investigation on the association between the Acanthamoeba genotypes and severity of the corneal infection”

Supervisors:

Dr Nicole Carnt, and Prof. Mark Willcox

Email Address: b.rayamajhee@unsw.edu.au

Linked in: https://www.linkedin.com/in/binod-rayamajhee-17b689127/

RESEARCH

Abstract

Acanthamoeba is a free-living opportunistic protozoan, which can cause a severe form of sight threatening infection of cornea, called Acanthamoeba keratitis (AK). AK is most common in contact lens wearers and is often misdiagnosed with other microbial keratitis such as herpes simplex virus keratitis. Early diagnosis and appropriate treatment on time is necessary to minimize the severity associated with AK. Study of 18S rRNA has revealed eighteen genotypes (T1-T18) of Acanthamoeba. Genotype T4 is predominantly associated with AK and shows high level of binding capacity to host cells. Therefore, genotyping should be an integral part of clinical diagnostic setting which may help to reduce the disease burden. Studies have shown that there is association between the Acanthamoeba genotypes and severity of the corneal infection. Genotyping of Acanthamoeba could add further insight in the diagnosis and may provide predictive value for clinical outcomes. Genetic analysis permits rapid diagnosis with high sensitivity which could play a significant role in specific anti-amoebic therapy.  

BIOGRAPHY

Binod completed his post-graduate study in Medical Microbiology from Tribhuvan University, Nepal in 2017. After completion of his post-graduate, Binod was extensively working in different organizations of Nepal focusing antimicrobial resistance (AMR) and infection control. Back in 2017, Binod co-founded a research institute in Nepal which works mainly in infectious diseases (https://kribs.org.np/) in national and international collaboration. The research interest of Binod includes AMR, viral pathogenesis, host defence mechanism, drug discovery, contact lens associated eye infections, disease surveillance, interspecies transmission of disease, and molecular diagnosis of infectious diseases. Binod practically focuses on how the surging burden of infectious diseases can be addressed locally and globally. The PhD project of Binod aims to identify the association between keratitis causing Acanthamoeba genotypes and disease severity of AK and/or clinical outcomes, and host defence mechanism against the AK will be examined.

Education

Year

Degree

Institution

Current

PhD Candidate

University of New South Wales, Australia

2017

MSc in Medical Microbiology

Tribhauvan University, Nepal

2013

BSc in Microbiology

Tribhauvan University, Nepal

RECENT PUBLICATIONS

Journal Articles

  • Maharjan S, Rayamajhee B, Shreshtha A, Acharya J. Serotyping and antibiotic susceptibility patterns of Vibrio and Shigella isolates from diarrheal patients visiting a Tropical and Infectious Diseases Hospital in central Nepal. BMC Research Notes. 2017 Dec 1;10(1):626. (10.1186/s13104-017-2967-0).
  • Upreti N, Rayamajhee B, Sherchan SP, Choudhari MK, Banjara MR. Prevalence of methicillin resistant Staphylococcus aureus, multidrug resistant and extended spectrum β-lactamase producing gram negative bacilli causing wound infections at a tertiary care hospital of Nepal. Antimicrobial Resistance & Infection Control. 2018 Dec 1;7(1):121. (https://doi.org/10.1186/s13756-018-0408-z).
  • Neupane K, Rayamajhee B, Acharya J, Rijal N, Shrestha D, GC B, Pant MR, Shah PK. Comparison of Nasal Colonization of Methicillin-Resistant Staphylococcus aureus in HIV-Infected and Non-HIV Patients Attending the National Public Health Laboratory of Central Nepal. Canadian Journal of Infectious Diseases and Medical Microbiology. 2018;2018. (https://doi.org/10.1155/2018/4508757). 
  • Maharjan S, Rayamajhee B, Chhetri VS, Sherchan SP, Panta OP, Karki TB. Microbial quality of poultry meat in an ISO 22000: 2005 certified poultry processing plant of Kathmandu valley. International Journal of Food Contamination. 2019 Dec;6(1):1-9. (https://doi.org/10.1186/s40550-019-0078-5).  
  • Bhandari S, Rayamajhee B, Dhungel L, Poudel S, Gaire B, Shrestha S, Parajuli N. Infectious sources of Histoplasmosis and molecular techniques for its identification. Nepal Journal of Biotechnology. 2019 Dec 29;7(1):96-102. (https://doi.org/10.3126/njb.v7i1.26949).

Posters and Abstracts

  • Rayamajhee B, Aacharya J, Ghimire L, ‘Methicillin-resistant Staphylococcus aureus in people living with HIV/AIDS visiting a Tropical and Infectious Disease Hospital in Nepal’. ASM microbe 2016, June 16-20, 2016, Boston, U.S.A. 
  • Rayamajhee B, Manandhar S, KC N, Parajuli P, Aacharya J, ‘Incidence of nalidixic acid resistant Salmonella enterica serovar Typhi and Salmonella enterica serovar Paratyphi A and their antibiotic susceptibility pattern isolated from enteric fever patients attending Hospital in Kathmandu’, Annual Conference of SfAM, 4 – 7 July 2016, The Assembly Rooms, Edinburgh, U.K. 
  • Rayamajhee B, Ghimire L, Aacharya J, Pun B, Maharjan S, Manandhar S, ‘Determining Serotypes and Antibiotic Susceptibility Patterns of Shigella and Vibrio Isolates from HIV-sero Positive Children Patients Attending National ART Reference Laboratory in Kathmandu’, asm microbe 2017, June 1-5, 2017, New Orleans, LA, U.S.A.
  • Rayamajhee B, Parajuli DP, Panta OP, Ghimire L, ‘Prevalence of foodborne pathogens in fresh, minimally-processed green vegetables and fruits and their drug resistant status from retail establishment of Kathmandu valley’, SfAM annual meeting, July 3-6, 2017, New Castle, U.K. 
  • Rayamajhee B, Manandhar S, Sherchand SP, Sherchan S, Aacharya J, ‘Prevalence of diarrheal disease in HIV positive children patients with their CD4 T-cells level in Kathmandu, Nepal’, 66th annual meeting of the American Society of Tropical Medicine and Hygiene, Nov 5-9, 2017, Baltimore, U.S.A. 

Reports and Book Chapters

  • Rayamajhee B, Sherchan S., ‘Prevention is Care’, Microbiologist Magazine, Society for Applied Microbiology, LABS, 90 High Holborn, London, UK. March, 2018, Page 14-17. Available: https://issuu.com/societyforappliedmicrobiology/docs/microbiologist_march_2018_web_82_re
  • Rayamajhee B, ‘Role of community pharmacists in controlling antimicrobial resistance’, Microbiologist Magazine, Society for Applied Microbiology, LABS, 90 High Holborn, London, UK. December, 2018, Page 51. Available: (https://issuu.com/societyforappliedmicrobiology/docs/sfam_microbiologist_december_2018_w).

AWARDS

  • 2020 - TFS plus a Research Stipend Scholarship from University of New South Wales, Sydney, Australia 2020-2024.
  • 2020 - Research Grant from Ministry of Economic Affairs and Planning, Province 3, Government of Nepal.
  • 2019, 2018 - Annual Conference Studentship from Society for Applied Microbiology (SfAM), LABS, 90 High Holborn, London, U.K.
  • 2018 - Public Engagement Grant from Society for Applied Microbiology (SfAM), LABS, 90 High Holborn, London, U.K.
  • 2017 - Research Support Grant from Nepal Academy of Science and Technology (NAST), Nepal.
  • 2017 - Bill and Melinda Gates Foundation Travel Award to attend the symposium organized by American Society of Tropical Medicine and Hygiene (ASTMH), U.S.A.
  • 2017 - ASM   Student   and   Postdoctoral   Travel   from   the   American   Society for Microbiology (ASM), U.S.A.
  • 2017, 2016 - Summer Conference Studentship from Society for Applied Microbiology (SfAM), LABS, 90 High Holborn, London, U.K.
  • 2016 - Conference Travel Assistantship (CTA) from Nepal Academy of Science and Technology (NAST), Kathmandu, Nepal.
  • 2016 – Post-graduate Research Support from National School of Science and Technology (NIST), Kathmandu, Nepal.
  • 2015-2016 - Graduate Student Research (GSR) grant from Childreach Nepal and UNICEF Nepal.

CONFERENCE ATTENDANCE

  • 2019 - 8th Congress of European Microbiologists, 7-11 July, 2019, Glasgow, Scotland.
  • 2018 - Annual meeting of Society for Applied Microbiology, July 9-12, 2018, Brighton, U.K.
  • 2018 - First Science, Information and Technology National Youth Conference (SIT-NYC, 2018), June 15-17, Nepal Academy of Science and Technology, Lalitpur, Nepal.
  • 2018 – Workshop organized by Kathmandu Center for Genomics and Research Laboratory (KCGRL) on ‘Genetic Approach to Blood Disorders, An Answer to Many Unanswered Question’, Feb 26, 2018.
  • 2017 - Academic symposium organized by U.S. Embassy in Nepal, December 13, 2017, Kathmandu, Nepal.
  • 2017 - 66th annual meeting of the American Society of Tropical Medicine and Hygiene (ASTMH), Nov 5-9, 2017, Baltimore, U.S.A.
  • 2017 - Third National Summit of Health and Population Scientists in Nepal. April 10-12, 2017, Kathmandu, Nepal. 
  • 2017 - Sixth Asia Pacific Conference of Young Scientists, Nov 1- 5, 2017, Park Village Resort, Budhanilkantha, Kathmandu, Nepal.
  • 2017 - ASM microbe 2017, June 1-5, 2017, New Orleans, LA, U.S.A.
  • 2017 – Summer Conference of Society for Applied Microbiology, July 3-6, 2017, New Castle, U.K.
  • 2016 – ASM microbe 2016, June 16-20, 2016, Boston, U.S.A.
  • 2016 – Annual Conference of Society for Applied Microbiology, 4 – 7 July 2016, The Assembly Rooms, Edinburgh, U.K.
  • 2016 - Second National Summit of Health and Population Scientists in Nepal. April 11-12, 2016, Kathmandu, Nepal.
  • 2016 - Symposium on ‘Microbiology and one health concept in Nepal’, September, 2016, Kathmandu, Nepal.
  • 2015 - Second International Conference on Infectious Diseases and Nanomedicine (ICIDN), Dec. 15-18, 2015, Kathmandu, Nepal.
  • 2015 - Public Engagement Workshop on Microbiology for Social Transformation, 2015, Kathmandu, Nepal. 
  • 2010 - Second International Symposium on Biotechnology-Education, Research & Industrialization. June 29-30, 2010, Kathmandu, Nepal.

AFFILIATIONS AND MEMBERSHIPS

  • Member: The Consortium of Universities for Global Health (CUGH), Washington DC, 20036, U.S.A.
  • Communication Officer: Microbiology Research Society (MRS), Kathmandu, Nepal.
  • Global Outreach Member: American Society for Microbiology (ASM), Washington DC, U.S.A.
  • International Representative, Early Career Scientist Group: Society for Applied Microbiology (SfAM), U.K.
  • Present: Micro-Club, National College, Kathmandu, Nepal.
  • Member: European Society for Clinical Microbiology and Infectious Disease (ESCMID), Basel, Switzerland.
  • Member: The American Society of Tropical Medicine and Hygiene, Arlington, VA 22202 U.S.A.
  • Community Ambassador: eLife Sciences Publications, Ltd, Westbrook Centre, Milton Road, Cambridge CB4 1YG, U.K.
  • Co-founder: Kathmandu Research Institute for Biological Sciences (KRIBS), Lalitpur, Nepal.