Research Title: Biosampling of Corneal Infiltrative Events for the Identification of Metabolites Expression
Supervisors: Dr Nicole Carnt, Dr Ajay Kumar Vijay, Prof Fiona Stapleton
Email Address: g. email@example.com
Contact lens wear is a major cause of corneal infiltrative events (CIEs), which present with different forms and severity. The incidence of contact lens related CIEs ranges as high as 6.0 % in symptomatic cases and 44% in asymptomatic cases per year. The present study aims to identify tear metabolites expressed during CIEs that can be used as biomarkers of ocular inflammation, and to explore the biological pathways associated with CIEs. Three major prospective studies will be carried out by collecting tear samples from the patients with CIEs at School of Optometry and Vision Science (SOVS), UNSW and WSLHD Westmead Hospital, Westmead and unaffected controls using Ultra-High-Performance liquid chromatography (UHPLC MS/MS) 1) untargeted metabolomic profiling of tear samples to identify biomarkers associated with CIEs; 2) targeted metabolomic study to confirm biomarkers identification and mining of biological pathways; and 3) validation study of biomarker characterisation. The raw data will be converted to a matrix of chromatographic peaks and will generate a peak table of m/z, retention time and alignment of raw chromatographic data to identify metabolites biomarkers. This study will be the first study to develop methods for the tear sampling and analysis using UHPLC MS/MS for the study of CIEs.
This study is believed to improve understanding of CL-related and non-CL- related infiltrative events to identify the risk groups for both onset and the severity of the disease, the pathway associated with the metabolic interaction and guide early intervention and prevention in future.
Mr Shrestha is an optometrist, who received B. Optom degree (2002) from Institute of Medicine, Nepal and M.Optom degree (2007) from Bharati Vidyapeeth University, India. He worked as a clinical optometrist and an optometry faculty at various capacities in Institute of Medicine, Tribhuvan University. He has joined PhD degree in 2018 as a Scientia PhD Scholar in the SOVS, UNSW.
- Master of Optometry (2007), Bharati Vidyapeeth University, India
- Bachelor of Optometry (2002), Institute of Medicine, Nepal
- The Human Proteomic Organization Congress Sep 30, to Oct 4, 2018, Orlando, USA
- Shrestha GS, Vijay AK, Stapleton F, Carnt N. The effect of collection method on tear interleukin-6 levels in healthy individuals: A pilot study. ARVO 2019; E-Abstract 5370.
AFFILIATIONS AND MEMBERSHIPS
Student member of
- The Human Proteomic Organization July 2018 to Dec 2020
- The Association for Research in Vision and Ophthalmology, 2019
- Cornea and Contact Lens Society of Australia, 2019