Honours Projects

Supervisors: Scientia Professor Fiona Stapleton (SOVS) Associate Professor Blanka Golebiowski (SOVS) Dr Minh Phan (SOVS) Dr Martin Bucknall (MWAC) 

Summary: This project seeks to develop novel methods to measure levels of a variety of sex hormones in human tears, serum and in tissue culture, using quantitative nano-LCMS stable isotope dilution. In one of their many roles in the human body, sex hormones (incl. androgens and oestrogens) are involved in production of the tear film which protects and nourishes the front of the eye. Funded by the Australian Research Council, our group is conducting research to understand how sex hormones regulate tear film production and thus maintain a healthy ocular surface and enable clear vision. Only small volumes of tears can be collected from human subjects, and currently available methods are not sufficiently sensitive to quantify sex hormone levels in tear samples. Our group has pioneered methods to detect sex hormones, and this honours project will help us to improve the sensitivity of these methods in order to analyse individual tear samples and extracts from tissue culture. The project will involve work in the School of Optometry and Vision Science (SOVS) and in the Mark Wainwright Analytical Centre (BMSF Laboratory), under joint supervision, and will collaborate with world-leading experts in both basic and applied science. Training in tandem mass spectrometry, nano-flow liquid chromatography and tear collection will be provided. The project would suit an enthusiastic student with an analytical background, chemistry / biochemistry wet lab skills and good attention to detail. A stipend will be available to the successful candidate.

For further information please contact Dr Minh Phan: m.phan@unsw.edu.au

We are taught the importance of frequent handwashing, but how well do we wash our hands? Currently, we lack an accessible tool for quantifying how well the hands have been washed, and we instead have been primarily relying on subjective observation or tests that do not provide immediate feedback. This project aims to validate and further develop an automated tool using augmented reality (AR) to measure the effectiveness of different handwashing procedures. The research will have widespread implications for improving community hygiene and health outcomes globally. 

For further information, please contact Dr Nayuta Yoshioka (n.yoshioka@unsw.edu.au) 

Supervisors: Dr Lisa Nivison-Smith, Dr Poppy Watson, Assoc. Professor Steven Most

The Amsler grid is a low-cost, simple, paper-based tool prescribed to individuals with early age-related macular degeneration (AMD) to self-monitor their central vision. Individuals are advised to quickly seek medical advice if they detect visual changes using the grid as this may indicate progression to late AMD and treatment for this stage is most effective when administered early.

A major shortcoming of the Amsler grid is low compliance with on 55% of patients using it regularly. Suggested improvements to compliance such as follow-up education or electronic reminder systems are resource heavy and at odds with the low-cost nature of the test.

This project will assess if concepts from health psychology can improve compliance including linking the test to a habitual behaviour (i.e. brushing teeth) or a cognitive reward (i.e. favourite photo). This could provide a highly translatable way to improve Amsler grid compliance.

For further information please contact Dr Lisa Nivison-Smith: l.nivison-smith@unsw.edu.au

Hypercholesterolaemia is associated increased risk of heart disease and stroke. Lack of clear symptoms however means most people with the disease remain undiagnosed.

Cholesterol also plays an important role in the retina and abnormalities in cholesterol is associated with eye diseases such as macular degeneration.

This study looks at the retinal structure of individuals with hypercholesterolaemia to see if there is evidence of changes similar to macular degeneration. This could possibly lead to eye-based detection methods for hypercholesterolaemia.

This project is a collaboration between Dr Lisa Nivison-Smith and Dr Helen Williams at Westmead Hospital.

For further information please contact Dr Lisa Nivison-Smith: l.nivison-smith@unsw.edu.au

Supervisors: Dr Lisa Nivison-Smith

Summary: Chronic hypertension (HTN) is strongly associated with cardiovascular disease and stroke. This risk is greater when HTN has also caused microvascular damage in the retina. Thus detecting hypertensive retinal vessel changes is critical to identifying those at greatest risk of systemic disease. 

OCT angiography (OCTA) is a new, rapid and non-invasive method to image retinal vasculature. Preliminary work using OCTA has shown reduced vessel density and perfusion in HTN however it is not known which retinal areas indicate damage early and should be monitored.

We recently developed high density OCTA cluster analysis which can reveal subtle, location-specific changes in retinal vasculature. This project will use the analysis to isolate early retinal changes in HTN.

For further information please contact Dr Lisa Nivison-Smith: l.nivison-smith@unsw.edu.au

Supervisors: Dr Lisa Nivison-Smith, Professor Michael Kalloniatis

Summary: Macular neovascularisation is a major reason for vision loss in age-related macular degeneration (AMD) and target of the only effective treatment. Several clinical trials show patients have better outcomes if we detect and treat neovascularisation early.

Evidence of choroidal changes in early AMD is conflicting with some suggesting thinning at the fovea and others suggesting no change. This may be due to assessing the choroid using thickness which will miss subtle changes in blood vessel architecture.

This project will assess the choroid in AMD using a novel tool - the choroidal vascularity index (CVI). CVI can indicate subtle changes in the size and shape of choroidal vessels not possible with thickness measurements. CVI will be quantified across the macula to create a map of the choroid angioarchitecture which can be used to detect those at greatest risk of vision loss.

For further information please contact Dr Lisa Nivison-Smith: l.nivison-smith@unsw.edu.au

Supervisors: Dr Jack Phu and Prof Michael Kalloniatis

Summary: Clinicians are confronted with a wealth of data obtained in an examination to make decisions on diagnosis and management plans. The requirement to integrate large volumes of data, especially in modern practice, can specifically confound the diagnosis of glaucoma, the leading cause of irreversible vision loss, from non-glaucomatous optic disease. By applying statistical and pattern recognition analysis, the project aims to develop models to explain these differences with the goal of not only directly guiding clinicians' behaviour in practice, but also to better understand the underlying pathophysiology of glaucoma and eye disease.

For further information please contact Dr Jack Phu: jphu@cfeh.com.au

Supervisor: Dr Juno Kim

Summary: You will join the Sensory Processes Research Laboratory to undertake research in the field of virtual reality and multisensory integration. You will be responsible for leading a research project examining how we rely on multisensory integration to perceptually infer the physical properties of real and simulated environments. The application of outcomes from this research will have broad implications for industries spanning health and entertainment. If you desire, you may also have an opportunity to engage with industry on collaborative research. This competitive research opportunity directly builds on your learning in the OPTM3201 course.

For further information please contact Dr Juno Kim: juno.kim@unsw.edu.au

Supervisors: Dr Jerome Ozkan, Prof Minas Coroneo

Cataract surgery is one of the most frequently performed surgical procedures in the world. Use of antiseptics and antibiotics as part of cataract surgery is necessary to minimise the risk of postoperative bacterial endophthalmitis. However, following successful cataract surgery, studies have reported an increase in post-operative dry eye syndrome and cataract surgery to be associated with dry eye. What impact the use of ocular antisepsis/antibiotic treatment during cataract surgery has on the ocular microbiome and the development of post-operative dry eye syndrome is currently not known. 

Using DNA gene sequencing, this study will address what effect ocular antisepsis/antibiotic use associated with cataract surgery has on the ocular surface microbial composition and whether any antibiotic induced perturbation in the ocular microbiota is correlated with changes in dry eye symptoms in patients.  

For further information please contact Dr Jerome Ozkan: j.ozkan@unsw.edu.au

Supervisors: Dr Parthasarathi Kalaiselvan, Dr Ajay Vijay, Prof Mark Willcox

Summary: As we all know, during COVID-19 there has been increased awareness of the transmission of SARS-CoV-2 via aerosol. Healthcare workers and the general public have been asked to wear face masks to try to limit the extent of transmission of the virus. There are been claims that simply wearing glasses can protect your eyes from infection.

Face masks have been made by many manufacturers, often ones that have no previous experience in this area, and most of these have not been investigated thoroughly. The claims of glasses helping to protect your eyes from infection with viruses has not been systematically investigated.

You will learn how to grow a mouse version of coronavirus, one that cannot infect humans. This virus is recognised by regulatory authorities and scientists as an appropriate surrogate for SARS-CoV-2. You will aerosolise the virus in a closed chamber. Using a mannequin head, you will examine whether glasses of various shapes and sizes can protect the eyes of the mannequin from the virus. You will also use a custom designed test rig that aerosolises the virus and allows you to test whether face masks can prevent the transmission of the virus in the aerosols through them.

For further information please contact Prof Mark Willcox: m.willcox@unsw.edu.au